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Genomics II: Bacteria, Viruses and Metabolic Pathways
Title
Genomics II: Bacteria, Viruses and Metabolic Pathways
Editor
iConcept Press
Price
USD$139.00
ISBN
978-1-480254-145
Clicks
22878

Chapter 13

Genomics II: Bacteria, Viruses and Metabolic Pathways

Essential Genes Identification in Streptococcus Sanguinis and Comparison among Streptococci

by Ping Xu and Xiuchun Ge

Viewed: 2085

Abstract

In order to discover drug targets that will control streptococci, essential genes in the pathogen need to be identified. Identification of these genes will also provide further insight into the origin of life. After finishing the Streptococcus sanguinis genome and annotating all of its genetic information, we established a method to systematically construct S. sanguinis gene replacement mutants. We created genome-wide deletion mutants along S. sanguinis genome. Every single gene in S. sanguinis genome was attempted to delete. During the creation of the mutants, a clear set of essential genes crucial to S. sanguinis survival under experimental conditions was identified. The metabolic/genetic pathways of these essential genes were studied as a whole by systems biology. Three basic biological function pathways associated with the essential genes were found, including maintenance of the cell envelope, energy production, and processing of genetic information. We experimentally examined several genes in Streptococcus pneumoniae and Streptococcus mutans, two other pathogenic streptococcal species, and further validated our finding on the essential gene associated metabolic/genetic pathways. We also investigated conservation of S. sanguinis essential genes among other streptococci. Gene comparisons among different streptococcal genomes indicated that essential gene sequences were significantly more conserved than other protein genes except three genes that encode: Serine/alanine adding enzyme (SSA_0862), glucose-6-phosphate 1-dehydrogenase (SSA_1555) and 6-phosphogluconate dehydrogenase (SSA_1811). Our experimental data produced a simplified model that facilitates accurate predictions of gene essentiality in streptococci as well as other bacterial species.

Author Details

Ping Xu
The Philips Institute, Virginia Commonwealth University, USA, USA
Xiuchun Ge
The Philips Institute, Virginia Commonwealth University, USA, USA

Citation

Ping Xu and Xiuchun Ge. Essential Genes Identification in Streptococcus Sanguinis and Comparison among Streptococci. In Genomics II: Bacteria, Viruses and Metabolic Pathways. ISBN:978-1-480254-145. iConcept Press. 0000.

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