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Genomics II: Bacteria, Viruses and Metabolic Pathways
Title
Genomics II: Bacteria, Viruses and Metabolic Pathways
Editor
iConcept Press
Price
USD$139.00
ISBN
978-1-480254-145
Clicks
22864

Chapter 7

Genomics II: Bacteria, Viruses and Metabolic Pathways

Retroviral Integration Selectivity

by Tatsuaki Tsuruyama and Rei Ohmori

Viewed: 1653

Abstract

Integration of the retroviral genome into host DNA is a critical step in the life cycle of a retrovirus. Although assays for in vitro integration have been developed, the actual DNA sequences targeted by murine leukemia retrovirus (MLV) and HIV-1 during in vitro reproduction are unknown. While previous studies used artificial target sequences, we developed an assay using target DNA sequences from common MLV integration sites in the genome of murine lymphomas and an HIV-1 integration site in the genome of T cell successfully integrated into the target DNA in vitro. Selected integration sites were also common integration site in vivo. This coincidence suggests that there are several integration hot spots. The approach described here can provide data on the biochemical properties of the integration reaction to which the target DNA structure may contribute.

Author Details

Tatsuaki Tsuruyama
Department of Molecular Pathology, Graduate School of Medicine, Kyoto University, Japan, Japan
Rei Ohmori
Department of Molecular Pathology, Graduate School of Medicine, Kyoto University, Japan, Japan

Citation

Tatsuaki Tsuruyama and Rei Ohmori. Retroviral Integration Selectivity. In Genomics II: Bacteria, Viruses and Metabolic Pathways. ISBN:978-1-480254-145. iConcept Press. 0000.

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