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Genomics II: Bacteria, Viruses and Metabolic Pathways
Title
Genomics II: Bacteria, Viruses and Metabolic Pathways
Editor
iConcept Press
Price
USD$139.00
ISBN
978-1-480254-145
Clicks
22871

Chapter 12

Genomics II: Bacteria, Viruses and Metabolic Pathways

Whole Genome Sequencing of Influenza C Virus

by Reinhard Vlasak and Karin Pachler

Viewed: 2699

Abstract

Influenza C virus is a member of the Orthomyxoviridae, a family comprising viruses with segmented single-stranded RNA genomes of negative polarity. The genome of influenza C virus is distributed among seven RNA segments, which code for nine viral proteins. An important feature of the RNA segments of influenza viruses are the noncoding regions at the 3\' and 5\' ends. These noncoding ends play a crucial role in viral RNA transcription and replication. A large number of different influenza C virus strains has been characterised. Influenza C viruses are divided into six genetic and antigenic groups. The sequences of single RNA segments or partial RNA segments of several different influenza C virus strains are available in public databases. But the sequences of all seven RNA segments including the important noncoding ends have only been deposited for three different influenza C virus strains so far. For RNA viruses, special techniques are required to obtain nucleotide sequences of the 5´- and 3´- ends. In this chapter, we describe a strategy that allowed us to determine the whole genome sequence of influenza C virus strain C/JJ/50, including the noncoding regions. We also summarise sequence analysis approaches for other strains, show methods for sequence alignments and compare the genomic sequences of different Influenza C virus strains.

Author Details

Reinhard Vlasak
Department of Molecular Biology, University Salzburg, Austria
Karin Pachler
Department of Molecular Biology, University Salzburg, Austria

Citation

Reinhard Vlasak and Karin Pachler. Whole Genome Sequencing of Influenza C Virus. In Genomics II: Bacteria, Viruses and Metabolic Pathways. ISBN:978-1-480254-145. iConcept Press. 0000.

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