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Genomics II: Bacteria, Viruses and Metabolic Pathways
Title
Genomics II: Bacteria, Viruses and Metabolic Pathways
Editor
iConcept Press
Price
USD$139.00
ISBN
978-1-480254-145
Clicks
22875

Chapter 15

Genomics II: Bacteria, Viruses and Metabolic Pathways

High-Throughput Genome Analysis for Structure-based Rational Drug Design: Comparative Genome Analysis and Protein Modeling

by Ana Carolina Ramos Guimarães, Priscila Vanessa da Silva Zabala Capriles, Antonio Basílio de Miranda, Wim M. Degrave and Laurent Emmanuel Dardenne

Viewed: 2205

Abstract

High-throughput genome analysis has been used in the identification of putative drug targets allowing a better understanding of the role played by enzymes that are responsible for the catalysis of biochemical reactions in metabolic pathways involved in a variety of diseases. Moreover, a detailed study of all genome enzymes can uncover new catalytic mechanisms, add information about the origin and evolution of biochemical pathways and reveal potential targets for drug development. For many diseases caused by parasites, therapeutic options remain inefficient or nonexistent, requiring the search for new drug targets. These targets may be proteins specific to the metabolic pathways of the parasite or biomolecules present in both organisms but with different three-dimensional structure, like analogous enzymes. Nevertheless, experimental tests with all target candidates could be impracticable. To avoid this, computational methods have been used in structure-based rational drug design to accelerate the steps of identification and comprehension of important molecular interactions between receptor and lead compounds. In this chapter we will present how the comparative genome analysis and protein modeling could help to find putative drug targets in parasite genomes, more specifically in the Trypanosoma cruzi genome responsible for Chaga\'s disease in humans. Our approach involves the use of the pipeline AnEnPi which is able to identify, annotate and compare homologous and analogous enzymes, and the workflow MHOLline that was used to obtain automatically three-dimensional models for homologous, analogous and specific proteins of T. cruzi versus Homo sapiens.

Author Details

Ana Carolina Ramos Guimarães
Laboratório de Genômica Funcional e Bioinformática, FIOCRUZ/RJ, Brazil, Brazil
Priscila Vanessa da Silva Zabala Capriles
Programa de Pós-graduação em Modelagem Computacional, Universidade Federal de Juiz de Fora, Brazil, Brazil
Antonio Basílio de Miranda
Laboratório de Biologia Computacional e Sistemas, FIOCRUZ/RJ, Brazil, Brazil
Wim M. Degrave
Laboratório de Genômica Funcional e Bioinformática, FIOCRUZ/RJ, Brazil, Brazil
Laurent Emmanuel Dardenne
Grupo de Modelagem Molecular de Sistemas Biológicos, Laboratório Nacional de Computação Científica, Brazil, Brazil

Citation

Ana Carolina Ramos Guimarães, Priscila Vanessa da Silva Zabala Capriles, Antonio Basílio de Miranda, Wim M. Degrave and Laurent Emmanuel Dardenne. High-Throughput Genome Analysis for Structure-based Rational Drug Design: Comparative Genome Analysis and Protein Modeling. In Genomics II: Bacteria, Viruses and Metabolic Pathways. ISBN:978-1-480254-145. iConcept Press. 0000.

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