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Genomics III: Methods, Techniques and Applications
Title
Genomics III: Methods, Techniques and Applications
Editor
iConcept Press
Price
USD$139.00
ISBN
978-1-922227-416
Clicks
17338

Chapter 5

Genomics III: Methods, Techniques and Applications

Application of a Real-Time qPCR Methodology to Identify Microorganisms as Indicators of Biogas Production During Anaerobic Digestion

by Deborah Traversi, Valeria Romanazzi and Giorgio Gilli

Viewed: 1802

Abstract

Biogas is a bio-fuel with a quiet energy value that is connected to its methane content. It is a renewable energy source and produced via anaerobic digestion of various organic materials. In reactors, anaerobic microorganisms can degrade the waste organic matter into two different products: digestate and biogas. If the microbial community is optimised, then the methane production rate could be improved, and the digestate could be used as a soil fertiliser. Among the microorganisms involved in the digestion, the methanogens are the major microbiologic group responsible for methane production. Other microorganisms such as sulphate reducing bacteria, sulphur compounds oxidizing bacteria and syntrophic acetogenic bacteria have a relevant role in the consequent quantity and quality of the biogas. In order to study the microbiologic equilibrium into the anaerobic reactor, we are discussing on the application of Real Time quantitative PCR to detect microbiological group searching functional target genes, in the reactor, during the digestion. The discussed data suggest a role of some microorganisms, especially some methanogens, as diagnostic tool in the digestion optimisation. The illustrated integrative tools - some clearly still in a development phase - can greatly enhance the insight in the anaerobic digestion system.

Author Details

Deborah Traversi
University of the Study of Turin, Italy
Valeria Romanazzi
University of the Study of Turin, Italy
Giorgio Gilli
University of the Study of Turin, Italy

Citation

Deborah Traversi, Valeria Romanazzi and Giorgio Gilli. Application of a Real-Time qPCR Methodology to Identify Microorganisms as Indicators of Biogas Production During Anaerobic Digestion. In Genomics III: Methods, Techniques and Applications. ISBN:978-1-922227-416. iConcept Press. 0000.

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