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Obesity Research
Title
Obesity Research
Editor
iConcept Press
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USD$49.00
ISBN
978-1-922227-67-6
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Chapter 5

Obesity Research

The Effect of Fluorocitrate at the Spinal Level in a Rat Monoarthritic Pain Model: Possible Modulatory Role of D-serine

by Mariana Quiroz-Munoz, Alejandro Hernandez, Teresa Pelisser and Claudio Laurido

Viewed: 1071

Abstract

During chronic pain, there is a glial reaction in the spinal cord characterized by hypertrophy, proliferation, and up-regulation of immunoregulatory molecules and other glia-to-neuron mediators. Many of these changes have also been observed in adjuvant-induced arthritic rats, a widely used animal model of human rheumatoid arthritis. One of these glial mediators is D-serine, a ligand for the glycine modulatory binding site of NMDA receptors, a receptor subtype that plays a key role in nociceptive transmission in the spinal cord during chronic pain. We studied the functional contribution of glial cells in the spinal C-reflex and wind-up by challenging them with 2 nmol intrathecal (i.t.) fluorocitrate (FC). Results showed that FC presents an antinociceptive effect in the wind-up for both normal and monoarthritic rats. This effect was followed until 120 minutes after the i.t. FC application, showing a maximum maintained decrease in the wind-up scores (meaning antinociception) of around 60% in the normal rats and around 40% in the monoarthritic rats at around 90-120 minutes. The injection of D-serine (300 ┬ÁM i.t.) at time 60 minutes in a different set of experiments showed a reverting in the antinociceptive effect in both normal and monoarthritic rats. For normal rats, there was a decrease in the antinociceptive effect of around 65%; for monoarthritic rats, around 99%. The C-reflex was not modified by the FC treatment in either normal or monoarthritic rats. The former due probably to the fact that the response at lower frequencies (enough to evoke the C-reflex) of the sural nerve electrical stimulation, involves the activation of AMPA/AMPA/Kainate receptors and not the N-methyl-D-aspartate (NMDA) receptors. We can conclude that the antinociceptive effect of FC is reverted by D-serine, indicating a possible modulatory effect of this gliotransmitter.

Author Details

Mariana Quiroz-Munoz
Laboratory of Renal Physiology, Faculty of Biological Sciences, Catholic University of Chile, Chile
Alejandro Hernandez
Department of Biology, Faculty of Chemistry and Biology, University of Santiago of Chile, Chile
Teresa Pelisser
ICBM, University of Chile, Chile
Claudio Laurido
Department of Biology, Faculty of Chemistry and Biology, University of Santiago of Chile, Chile

Citation

Mariana Quiroz-Munoz, Alejandro Hernandez, Teresa Pelisser and Claudio Laurido. The Effect of Fluorocitrate at the Spinal Level in a Rat Monoarthritic Pain Model: Possible Modulatory Role of D-serine. In Obesity Research. ISBN:978-1-922227-67-6. iConcept Press. 2016.

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