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Protein Purification: Principles and Trends
Title
Protein Purification: Principles and Trends
Editor
iConcept Press
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USD$89.00
ISBN
978-1-922227-40-9
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20054

Chapter 2

Protein Purification: Principles and Trends

Haptoglobin and Apolipoprotein A-I as Biomarkers of High Altitude Pulmonary Edema

by Yasmin Ahmad, Narendra K. Sharma and Manish Sharma

Viewed: 2123

Abstract

We have investigated the plasma proteome by using 2D gel electrophoresis and MALDI TOF/TOF from patients with high altitude pulmonary edema (HAPE). A complete proteomic analysis was performed on 20 patients with HAPE and 10 healthy sea level controls. In total, we have identified 25 protein spots in human plasma and found that 14 of them showed altered changes in HAPE patients, which mainly were acute phase proteins (APPs), compliment components and apolipoproteins among others. Among the APPs, haptoglobin α2 chain, haptoglobin β chain, transthyretin and plasma retinol binding precursor showed overexpresion in HAPE patients as compared to controls. To validate the result of proteomic analysis, 2 proteins were selected for ELISA and western blotting analysis. Our data conclusively shows that two proteins, haptoglobin and apolipoprotein A-I are up-regulated in plasma of HAPE patients. These proteins may provide a fast and effective control of inflammatory damage until the subsequent mechanisms can begin to operate. Taken together, our findings further support the hypothesis that inflammatory response system is linked to the pathophysiology of HAPE.

Author Details

Yasmin Ahmad
Peptide and Proteomics Division, Defence Institute of Physiological & Allied Science (DIPAS), Delhi, India
Narendra K. Sharma
Peptide and Proteomics Division, Defence Institute of Physiological & Allied Science (DIPAS), Delhi, India
Manish Sharma
Peptide and Proteomics Division, Defence Institute of Physiological & Allied Science (DIPAS), Delhi, India

Citation

Yasmin Ahmad, Narendra K. Sharma and Manish Sharma. Haptoglobin and Apolipoprotein A-I as Biomarkers of High Altitude Pulmonary Edema. In Protein Purification: Principles and Trends. ISBN:978-1-922227-40-9. iConcept Press. 2016.

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