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Protein Purification: Principles and Trends
Title
Protein Purification: Principles and Trends
Editor
iConcept Press
Price
USD$89.00
ISBN
978-1-922227-40-9
Clicks
20059

Chapter 8

Protein Purification: Principles and Trends

Trends in Bone Tissue Engineering: Proteins for Osteogenic Differentiation and the Respective Scaffolding

by Vera Grotheer, Margit Schulze and Edda Tobiasch

Viewed: 1899

Abstract

Bone regeneration and replacement is a major focus in regenerative medicine since degenerative diseases and tumor surgery as well as accidents or dangerous recreational behavior is leading to an increasing need for bone reconstruction strategies. Especially for critical size bone defects, tissue engineering with mesenchymal stem cells is extensively studied because these cells are functioning as precursors for osteoblast in vivo. Nevertheless to reproduce the complex interaction of various factors in vitro is not an easy approach and further investigations have to be done. The status quo is summarized. A variety of growth and transcription factors are known to be involved in osteogenesis with bone morphogenetic proteins (BMPs) and the transcription factor Runx2 being the most extensively studied ones. But also PPAR γ and Osterix are generally regarded as the master regulators of osteoblast differentiation. Recently the large family of purinergic receptors has proven to be essential molecules in osteogenesis as well. In addition, scaffolding is needed to create a three-dimensional tissue. Recent developments in scaffold design are summarized, including natural and synthetic materials with or without the use of bioactive molecules constructed to mimic the natural environment. The status quo of scaffold fabrication methods such as 3D nanoprinting and their influence on cell-scaffold interactions is discussed. In this review we summarize the most interesting results and our related work focusing on two joined approaches: 1) the complex interaction of the most promising factors improving or accelerating osteogenic differentiation and ii) the development of scaffold materials with osteoconductive and osteoinductive properties.

Author Details

Vera Grotheer
Department of Trauma and Hand Surgery Medical Faculty, University Hospital Düsseldorf, Germany
Margit Schulze
Department of Natural Sciences, University of Applied Sciences Bonn-Rhine-Sieg, Germany
Edda Tobiasch
Department of Natural Sciences, University of Applied Sciences Bonn-Rhine-Sieg, Germany

Citation

Vera Grotheer, Margit Schulze and Edda Tobiasch. Trends in Bone Tissue Engineering: Proteins for Osteogenic Differentiation and the Respective Scaffolding. In Protein Purification: Principles and Trends. ISBN:978-1-922227-40-9. iConcept Press. 2016.

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