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Protein Purification: Principles and Trends
Title
Protein Purification: Principles and Trends
Editor
iConcept Press
Price
USD$89.00
ISBN
978-1-922227-40-9
Clicks
20051

Chapter 12

Protein Purification: Principles and Trends

Purification of PEGylated Proteins

by Mina Sepahi, Shahin Haddadian, Dariush Norouzian and Reza Ahangari Cohan

Viewed: 3926

Abstract

Conjugation of therapeutic proteins through polyethylene glycol technology causes increase in half life circulation of such drug in the body. But the adverse effect could be loss of pharmacokinetic properties of the drug under investigation. Proteins can be either randomly or selectively pegylated. However, the pegylated form of protein must be separated from the unpegyalted one which can be achieved by size exclusion, ion exchange and hydrophobic interaction chromatagraphies depending on the strategy employed.

Author Details

Mina Sepahi
Recombinant Biopharmaceuticals Production Department, Production and Research Complex, Pasteur Institute of Iran, Iran
Shahin Haddadian
Research and Development Department, Production and Research Complex, Pasteur Institute of Iran, Iran
Dariush Norouzian
Pilot Nano Biotechnology Department, Pasteur Institute of Iran, Iran
Reza Ahangari Cohan
Pilot Nano Biotechnology Department, Pasteur Institute of Iran, Iran

Citation

Mina Sepahi, Shahin Haddadian, Dariush Norouzian and Reza Ahangari Cohan. Purification of PEGylated Proteins. In Protein Purification: Principles and Trends. ISBN:978-1-922227-40-9. iConcept Press. 2016.

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