iConcept Press Logo
Email Password Remember me
Protein Purification: Principles and Trends
Title
Protein Purification: Principles and Trends
Editor
iConcept Press
Price
USD$89.00
ISBN
978-1-922227-40-9
Clicks
20056

Chapter 9

Protein Purification: Principles and Trends

Intracellular Tau Modifications and Cell-based Sensors for Monitoring Tau Aggregation

by Md. Mamunul Haque, Sungsu Lim, Dohee Kim, Dong Jin Kim and Yun Kyung Kim

Viewed: 2604

Abstract

Tau is a neuron-specific microtubule-binding protein that stabilizes microtubules (Kolarova et al., 2012). When pathologically modified, tau dissociates from microtubules and becomes insoluble aggregates called neurofibrillary tangles (NFTs). NFTs are accumulated in neuronal perikarya or dystrophic neurites in axons and dendrites, causing degeneration of tangle-bearing neurons. The NFT formation is one of the most significant pathological signatures in Alzheimer’s Disease (AD) and multiple neurodegenerative disorders classified as tauopathies. Accordingly, great efforts have been made to investigate the mechanism of tau aggregation and to identify the pathogenic tau species. Accumulating evidences suggest that soluble tau oligomers, rather than large insoluble aggregates, are the pathogenic forms responsible for neuronal degeneration and cognitive impairment. Formation of the fibrillary inclusions might serve as a neuronal defense mechanism to quarantine the toxic oligomers. In addition, tau oligomers propagate in neurons acting as a seed for native tau aggregation. Due to the pathological implication, tau oligomers become an important therapeutic target to cure tauopathies. However, progress has been slow due to the lack of understating tau aggregation mechanism. Tau is an intrinsically soluble protein. To become a neurotoxic aggregate, tau undergoes a number of harmful modifications. In this book chapter, we will look for various tau modifications associated with aggregation and also introduce cell models for monitoring tau aggregation in cells.

Author Details

Md. Mamunul Haque
Biological Chemistry, University of Science and Technology, Korea; Center for Neuro-medicine, Korea Institute of Science and Technology, Korea
Sungsu Lim
Center for Neuro-medicine, Korea Institute of Science and Technology, Korea
Dohee Kim
Department of Biotechnology, Translational Research Center for Protein Function Control, College of Life Science and Biotechnology, Yonsei Unviersity, Republic of Korea; Center for Neuro-medicine, Korea Institute of Science and Technology, Republic of Korea
Dong Jin Kim
Center for Neuro-medicine, Korea Institute of Science and Technology, Korea
Yun Kyung Kim
Biological Chemistry, University of Science and Technology, Korea; Center for Neuro-medicine, Korea Institute of Science and Technology, Korea

Citation

Md. Mamunul Haque, Sungsu Lim, Dohee Kim, Dong Jin Kim and Yun Kyung Kim. Intracellular Tau Modifications and Cell-based Sensors for Monitoring Tau Aggregation. In Protein Purification: Principles and Trends. ISBN:978-1-922227-40-9. iConcept Press. 2016.

Download