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Protein Purification: Principles and Trends
Title
Protein Purification: Principles and Trends
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iConcept Press
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USD$89.00
ISBN
978-1-922227-40-9
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20062

Chapter 5

Protein Purification: Principles and Trends

Single DNA Molecule Sensing with Boron Nitride Nanopores

by Zhi Zhou, Ying Hu, Xinyan Shan and Xinghua Lu

Viewed: 2551

Abstract

Ultra-thin solid-state nanopores with good wetting property are strongly desired for label-free DNA sequencing application. Recently, nanopores fabricated in atomic thick two-dimensional materials such as graphene, boron nitride, and molybdenum disulfide membranes, have been utilized to detect single DNA molecules. Among these ultra-thin nanopores, boron nitride nanopores are notable due to their high oxidization-resistivity, which allows the nanopores to be treated with UV-Ozone cleaning to improve their hydrophilicity. In this chapter, we introduce the fabrication process of boron nitride nanopores and demonstrate the conductance change in ionic current due to the translocation of both dsDNA and ssDNA through the nanopore. The hydrophilicity and 1/f noise performance of boron nitride nanopores were further characterized. Compared with graphene nanopores, the contact angle of boron nitride nanopores can be reduced from 57° to 26o by UV-Ozone treatment; and the 1/f noise is found to be related to the area of suspended boron nitride membranes. Born nitride nanopores with diameter less than two nanometers are fabricated with a yield over 50%. Such small size nanopores are expected to improve the signal sensitivity for single DNA molecule translocation. Abundant DNA translocation events have been observed in such devices, and strong DNA-nanopore interaction has been revealed in pores smaller than 10 nm in diameter. The demonstrated results open a window for DNA sensing with boron nitride nanopores and a potential platform for future DNA sequencing application.

Author Details

Zhi Zhou
Beijing National Laboratory for Condensed-Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, China; Collaborative Innovation Center of Quantum Matter, Beijing, China
Ying Hu
Beijing National Laboratory for Condensed-Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, China; Collaborative Innovation Center of Quantum Matter, Beijing, China
Xinyan Shan
Beijing National Laboratory for Condensed-Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, China; Collaborative Innovation Center of Quantum Matter, Beijing, China
Xinghua Lu
Beijing National Laboratory for Condensed-Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, China; Collaborative Innovation Center of Quantum Matter, Beijing, China

Citation

Zhi Zhou, Ying Hu, Xinyan Shan and Xinghua Lu. Single DNA Molecule Sensing with Boron Nitride Nanopores. In Protein Purification: Principles and Trends. ISBN:978-1-922227-40-9. iConcept Press. 2016.

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