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Research on Immunology
Title
Research on Immunology
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iConcept Press
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978-1-922227-91-1
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Chapter 7

Research on Immunology

Human Papillomavirus (HPV) 16 and 18 Variants in Northern Spain

by Laila Sara Arroyo, Nerea Fontecha Urcelay, Miren Basaras Ibarzabal, Elixabete Arrese Arratibel, Silvia Hernáez Crespo, Daniel Andía Ortiz and Ramón Cisterna Cancer

Viewed: 2012

Abstract

Human papillomavirus (HPV) variants differ in their biological and chemical properties, and therefore, may present differences in pathogenicity. Most authors classified variants based on the phylogenetic analysis of L1 region, defining some nucleotide substitutions that may interfere with the functional or antigenic properties of specific viral proteins. We aimed to characterize the L1 genetic variability of HPV 16 and 18 genotypes, working with both single infection and multiple HPV infection specimens. Furthermore, we wished to assess the prevalence of HPV variants in our region as well as to analyze the relationship between these variants and the type of lesion. From 2007 to 2010, Clinical Microbiology and Infection Control Department analyzed 143 samples which were positive for HPV 16 and/or 18. Genetic variability was determined in PCR products and variants were assigned to European, Asian-american/Asian-amerindian or African lineages. Association of variants, infection type and specific genotypes (besides HPV 16 and 18) with different types of lesion was studied. Genetic analysis of the regions revealed a total of 27 nucleotide variations, 10 of which being non-synonymous. Both genotypes showed the same variant distribution, being European variants the most prevalent variants (91/100 for HPV 16 isolates and 25/43 for HPV 18 specimens), followed by the African (6/100 and 10/43) and the Asian-american/Asian-amerindian variants (3/100 and 5/43 isolates, respectively). L1 nucleotide variability analysis for HPV 18 showed the impossibility to classify Asian-amerindian and European variants properly, as L1 sequences from most non-african isolates were 100% identical and therefore, other regions sequences had to be included to the analysis (E6, E7, E4 and URR). Analysis of all sequences detected the presence of recombination in 2/43 HPV isolates (4.7%). Samples taken from cervical cancer and high-grade squamous intraepithelial lesions (H-SIL) only presented variants with specific-african substitutions. Multiple HPV infection, non-european HPV variants prevalence and existence of recombination are considered risk factors for HPV persistence and progression of intraepithelial abnormalities, and therefore, should be taken into consideration in order to help to design and optimize diagnostics protocols as well as to improve epidemiologic studies. Our study is one of the few studies in Spain which analyses the genetic variability of both HPV 16 and HPV 18 and we showed the importance of characterizing more than one genomic region in order to detect recombination and classify HPV variants properly.

Author Details

Laila Sara Arroyo
Laboratory Medicine, Karolinska Institutet, Sweden
Nerea Fontecha Urcelay
Immunology, Microbiology and Parasitology Department, School of Medicine, University of Basque Country, Spain
Miren Basaras Ibarzabal
Immunology, Microbiology and Parasitology Department, School of Medicine, University of Basque Country, Spain
Elixabete Arrese Arratibel
Immunology, Microbiology and Parasitology Department, Faculty of Pharmacy, University of Basque Country, Spain
Silvia Hernáez Crespo
Clinical Microbiology and Infection Control Department, Basurto University Hospital, Spain
Daniel Andía Ortiz
Department of Obstetrics and Gynecology, University of Basque Country, Basurto University Hospital, Spain
Ramón Cisterna Cancer
Clinical Microbiology and Infection Control Department, University of Basque Country, Basurto University Hospital, Spain

Citation

Laila Sara Arroyo, Nerea Fontecha Urcelay, Miren Basaras Ibarzabal, Elixabete Arrese Arratibel, Silvia Hernáez Crespo, Daniel Andía Ortiz and Ramón Cisterna Cancer. Human Papillomavirus (HPV) 16 and 18 Variants in Northern Spain. In Research on Immunology. ISBN:978-1-922227-91-1. iConcept Press. 2016.

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